Figure 7.

Hypoxia-Induced Upregulation of HE4 Is Required for Resistance of GC to Radiation Therapy Both In Vitro and In Vivo

(A and B) Viability of GC cell lines SGC-7901 (A) and MKN-49P (B) stably expressing control or WFDC2 shRNA, bearing either empty vector or HIF1α overexpression (OE), respectively, after treatment with radiation dose as indicated, was assessed by clonogenic assay. (C and D) Mice (n = 6 each group) bearing SGC-7901 (C) and MKN-49P (D) xenograft tumors stably expressing control or WFDC2 shRNA, bearing either empty vector or HIF1α OE, respectively, were subjected to 10 Gy of radiation on days 15 and 20 after inoculation, and sizes of xenograft tumors were measured on indicated days. Data are presented as mean ± SD. *p < 0.05, and **p < 0.01, HIF1α OE + control versus empty vector + control and HIF1α OE + WFDC2 shRNA.