Fig. 5.

S-nitrosation of KLF4 in ET-1 stimulated ECs. (A) HUVECs were treated with ET-1 (0.1 μmol/L, 1 h). Immunofluorescence was done with antibody against S-nitrosocysteine (SNO). Nuclei were counterstained with DAPI. Rabbit IgG was used as negative control. *p < 0.05, by unpaired Student's t-test, n = 3. MFI, mean fluorescence intensity. (B) Cells were exposed to ET-1 (0.1 μmol/L, 1 h) with or without pretreatment with NAC (10 mmol/L, 3 h) or Bosentan (10 μmol/L, 1 h). Cell lysate was subjected to IBP to detect S-nitrosation of KLF4. *p < 0.05, by one-way ANOVA. n = 3.