Fig. 2.

Cd²⁺induces intracellular SOD1 oxidation. (a) Overlay of in-cell ¹H–¹⁵N NMR spectra acquired on cells expressing [U-¹⁵N] labelled SOD1 in defect of Zn²⁺ either untreated (black) or treated (red) with Cd²⁺; (d) overlay of in vitro¹H–¹⁵N NMR spectra acquired on disulfide-reduced E,Zn-SOD1 (blue) and disulfide—oxidised E,Zn-SOD1 (orange); (b, c, e, f) enlarged areas of the same spectra (dotted rectangles in a, c). Representative signals affected by the formation of the C57-C146 disulfide bond are labelled.