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. 2018 Dec 14;14:287–300. doi: 10.1016/j.omtn.2018.12.003

Figure 4.

Figure 4

PTEN Activation Inhibits Migration and Confers Increased Sensitivity to Combined B-Raf and PI3K/mTOR Inhibition in the SK-MEL-28 Melanoma Cell Line

dCas9-VPR was stably expressed in SK-MEL-28 with no sgRNA, PTEN sgRNA −54, or a mix of four sgRNAs targeting the PTEN proximal promoter. (A and B) Representative images of migrating SK-MEL-28 cells are displayed (A), along with quantification of the number of migrating cells relative to dCas9-VPR with no sgRNA (B). *p < 0.05, **p < 0.01; n = 3; error bars show SEM. (C–H) Long-term colony formation assay with SK-MEL-28 cells grown in dabrafenib (C and D), dactolisib (E and F), or combined dabrafenib and dactolisib (G and H) for a period of 3 weeks. Representative images of wells are displayed (C, E, and G), along with the average number of colonies per well (relative to dCas9-VPR with no sgRNA) and average colony diameter (D, F, and H). *p < 0.05, **p < 0.01; n = 3; error bars show SEM.