Figure 1.

Flow cytometry analysis of ALDH activity in cells isolated from a human cardiac atrial appendage tissue specimen using the Aldefluor™ assay. (a) ALDH^br gating is established by incubating Aldefluor™-reacted cells with the ALDH inhibitor DEAB (negative control). An ALDH/side scatter (SSC) plot is shown. ALDH^br gating was set to include the top 0.05% of DEAB-treated cells with respect to the intensity of the fluorescent signal. (b) Aldefluor-reacted cells analyzed in the absence of DEAB treatment.