Skip to main content
. Author manuscript; available in PMC: 2019 Jan 28.
Published in final edited form as: Nat Immunol. 2018 Jun 20;19(7):766–775. doi: 10.1038/s41590-018-0137-8

Fig. 1 ∣. Itch interacts with WWP2 through its third WW domain.

Fig. 1 ∣

a, Immunoblot analysis of HEK293T cells transfected with expression vectors for Xpress-tagged Itch and/or Myc-tagged WWP2 or not (above lanes), assessed after immunoprecipitation (IP) with anti-Xpress (α-Xpress) or no immunoprecipitation (Total lysate) (left margin); right margin, molecular size in kilodaltons (throughout). b, Immunoblot analysis of the interaction of endogenous Itch with WWP2 in Jurkat T cells, assessed after immunoprecipitation with anti-Itch or the control antibody IgG (above lanes; left) or no immunoprecipitation (right). c, Confocal microscopy of the intracellular localization of Xpress-tagged Itch and Myc-tagged WWP2 in NIH3T3 cells transfected to express those molecules. Scale bars, 20 μm. d, Full-length (WT) Itch and its deletion mutants lacking functional domains (left; ranges (left margin) indicate amino acids present in construct), and immunoblot analysis of the interaction of Itch and WWP2 in HEK293T cells co-transfected (above lanes) to express Myc-tagged WWP2 plus empty vector (EV) or Xpress-tagged full-length Itch or various deletion mutants (middle and right), assessed after immunoprecipitation with anti-Myc (middle) or no immunoprecipitation (right). Data are representative of two to four independent experiments.