Figure 2.
Representative flow cytometry plots of lung single-cell suspensions from the control, melatonin, and radiation-treated mice. (a) The gating strategy of CD45+ myeloid lung cells for characterization of pulmonary populations, such as infiltrating macrophages (CD11b+CD11c−) and alveolar macrophages (CD11b int CD11c+). Percentages of F4/80+CD206+ AM macrophage subsets (b) and F4/80+CD206+ infiltrating subsets (c) were statistically analyzed. (d) Percentages of CD11b+Ly6G+ neutrophils within CD45+ cells in the lungs were statistically analyzed. Means ± SEM are shown (n = 6 in each group). ∗ p < 0.01 compared with the control group. # p < 0.05 compared with the radiation group. Mel, melatonin; IM, interstitial macrophage; AM, alveolar macrophage.