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. Author manuscript; available in PMC: 2019 Jan 28.
Published in final edited form as: J Cell Biochem. 2018 Jul 16;119(11):8830–8840. doi: 10.1002/jcb.27133

Figure 3. Conditional overexpression of Mdm2 in osteogenic cells increases osteonectin, Sp1, and mineralization.

Figure 3.

A. (Top panel) Western Blot analysis demonstrating elevated Mdm2 levels in human MG63 cells compared to wild type controls and (Bottom panel) decreased Mdm2 levels in silenced Mdm2 MG63 cells compared to vector control. B. (Top panel) Western blot analysis demonstrate elevated levels of osteonectin in mouse cells with overexpression of Mdm2 protein levels compared to vector control and (Bottom panel) elevated levels of osteonectin with increasing Mdm2 levels. C. (Top panel) Elevated Sp1 protein levels in pLKO vector control MG63 cells compared to ShMdm2 MG63 cells. (Bottom panel) Elevated Sp1 protein levels in Mdm2 overexpressed MG63 cells compared to pLV vector control. D. Mineralization by Alizarin Red staining. (Top panel) Mouse calvarial OB Mdm2TgOb shows increased mineralization compared to wild type OB control. (Middle panel) Overexpression of Mdm2 in MG63 human osteosarcoma cells resulted in greater mineralization compared to empty pLV vector control. (Bottom panel) Silencing of Mdm2 (ShMdm2) in MG63 human osteosarcoma cells resulted in significantly less mineralization compared to empty pLKO vector control. Asterisk signifies statistical significance at p<0.05.