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. Author manuscript; available in PMC: 2019 Jan 28.
Published in final edited form as: J Mol Biol. 2008 Aug 6;382(5):1195–1210. doi: 10.1016/j.jmb.2008.07.088

Figure 8. X-Ray Crystal Structure of Human GAPDH with BisANS Incorporation Sites Modeled in NTg, G93A, and H46R/H48Q Mice.

Figure 8.

Figure 8.

BisANS cross-linked sites found by LC-MS/MS in mouse GAPDH are indicated in the stereoview of the crystal structure of tetrameric human GAPDH (pdb code 1u8f) described by Jenkins and Tanner51 followed by the corresponding human sites in parentheses. BisANS sites incorporated to GAPDH are shown only on one of the four subunits corresponding to the mouse amino acid number A) BisANS sites found in the NTg mouse GAPDH by LC/MS/MS are indicated by their mouse amino acid number. Amino acids involved in enzyme activity are indicated by black dots, and sites lost by both mutants are indicated by green, lost only in G93A by red, and only in H46R/H48Q in orange. B) Sites gained in G93A are indicated by red spheres, and those gained by both G93A and H46R/H48Q are indicated in cyan.