Figure 2.

1,25-(OH)₂D₃ inhibited the motility and invasive potential of breast cancer cells.

Notes: Breast cancer cells were treated with 1,25-(OH)₂D₃ in different concentrations or 0.1 µm tamoxifen (TAM). (A) Cell migration was determined using wound healing migration assay (×40). (B) The width of wound was measured using a microscope. (C) Numbers of invading cells were determined by counting using a microscope. (D) MCF-7 and MDA-MB-453 cells were plated in upper compartments of Matrigel invasion chambers and exposed to 1,25-(OH)₂D₃ or TAM. Then, invasive potential of treated cells was evaluated microscopically. Data are mean ± SD of three independent experiments. Statistical analysis of the relative migration index (E) and invaded cell numbers (F) of MCF-7 and MDA-MB-453 cells. *P<0.05 vs Blank, **P<0.01.

Abbreviation: 1,25-(OH)₂D₃, 1,25-dihydroxy vitamin D₃.