Figure 5.

The proliferation of 1,25-(OH)₂D₃-treated breast cancer cells was abrogated by overexpression of Ras.

Notes: Breast cancer cells were treated with 1,25-(OH)₂D₃ alone or together with the overexpression of Ras by using plasmid for 48 hours. (A) The expression of Ras protein in MCF-7 and MDA-MB-453 cells transfected with Ras-overexpressing plasmids was determined by Western blotting after the cells were treated for 48 hours. (B) The CCK-8 assay was used to determine the cell proliferation of MCF-7 cells every 24 hours for 2 days. (C) The CCK-8 assay was used to determine the cell proliferation of MDA-MB-453 cells every 24 hours for 2 days. (D) The expression of Ki67 protein in MCF-7 cells was determined by Western blotting after the cells were treated for 48 hours. (E) The expression of Ki67 protein in MDA-MB-453 cells was determined by Western blotting after the cells were treated for 48 hours. Error bars represent the SD. **P<0.01 vs Control, ^#P<0.05 vs 0.1 µm.

Abbreviations: 1,25-(OH)₂D₃, 1,25-dihydroxy vitamin D₃; CCK-8, cell counting kit-8.