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Activation of the Ras/MEK/ERK signaling was abrogated by Ras overexpression.
Notes: Breast cancer cells were treated with 1,25-(OH)2D3 alone or together with the overexpression of Ras by using plasmid for 48 hours. The protein levels of Ras and the phosphorylation of MEK and ERK1/2 in MCF-7 cells (A) and MD-MB-453 (B) cells were shown by Western blotting. The expression of GAPDH was used as an internal control.
Abbreviation: 1,25-(OH)2D3, 1,25-dihydroxy vitamin D3.
