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. 2019 Feb;25(2):247–254. doi: 10.1261/rna.068460.118

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© 2019 Ying et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Inhibition of EF-G-dependent translocation by Neo in polymix buffer. Shown is the extent of translocation (Fraction POST) in control and mutant ribosomes (as indicated) plotted against the concentration of Neo. Data were fit to a modified dose response equation, yielding the following micromolar IC₅₀ values (mean ± SEM, n ≥ 3): Control, 3 ± 0.4; A1408G, 45 ± 10; ΔH69, 15 ± 3; A1408G/ΔH69, 130 ± 30.