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. 2019 Feb;25(2):264–276. doi: 10.1261/rna.068106.118

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© 2019 Morita and Aiba; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 5. — (A) Effect of expression of the plasmid borne hfq-His₆ gene on cell growth. Overnight cultures of TM589 (Δhfq) cells harboring indicated plasmids were inoculated (1/1000-fold) into LB medium supplemented with indicated concentrations of IPTG. Incubation was continued for 300 min by measuring A₆₀₀. (B) Effect of expression of the plasmid borne mutated hfq-His₆ gene on cell growth. Overnight cultures of TM589 (Δhfq) cells harboring indicated plasmids were inoculated (1/1000-fold) into LB medium supplemented with 1 mM IPTG. Incubation was continued for 300 min by measuring A₆₀₀. (C) The rim mutation suppresses growth inhibition caused by the distal mutation. Overnight cultures of TM589 (Δhfq) cells harboring indicated plasmids were inoculated (1/1000-fold) into LB medium supplemented with 1 mM IPTG. Incubation was continued for 300 min by measuring A₆₀₀.