Fig. 2. FRET response of A-1 to Zn(ii) and proposed structures of metal-free and Zn(ii)-bound A-1. (a) Change in fluorescence upon incubation of A-1 (black) with Zn(ii) (green). Conditions: [A-1] = 0.5 μM; [ZnCl2] = 100 μM; λex = 280 nm. (b) Proposed structures of metal-free A-1 (left) and Zn(ii)-bound A-1 (right). The structures were generated by modifications of the previously reported structures of metal-free Aβ (PDB: ; 1AMC)27 and Zn(ii)-bound Aβ (PDB:; 1ZE9).28 The approximate distances between the FRET donor and acceptor were indicated with dashed lines.
