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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Nat Protoc. 2018 Aug;13(8):1829–1849. doi: 10.1038/s41596-018-0020-y
Initial denaturation 98 °C for 30 seconds
Denature 98 °C for 10 seconds Repeat for 5–18 cycles
Anneal 60 °C for 30 seconds
Extend 72 °C for 30 seconds
Final extention 72 °C for 5 minutes