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. Author manuscript; available in PMC: 2019 May 28.
Published in final edited form as: Nature. 2018 Nov 28;564(7735):278–282. doi: 10.1038/s41586-018-0750-6

Extended Data Fig. 2: NP220 and SETDB1 are required for the silencing of unintegrated MLV DNA.

Extended Data Fig. 2:

a, b, Hela (a) or NIH3T3 (b) cells were transfected with indicated siRNAs and then infected with VSV-G pseudotyped, integrase-deficient (IND184A) MLV-Luc virus. Luciferase activities were measured 40 h post infection and luciferase activity in non-targeting (NT) control siRNA transfected cells was set as 1 (top panels). Data presented are mean ± SD from three independent experiments (n = 3). The expression of NP220 was determined by Western blot (bottom panels). c, Hela cells were first transfected with indicated siRNAs targeting histone methyltransferases and then infected with VSV-G pseudotyped, integrase-deficient (IND184A) MLV-Luc virus. Luciferase activities were measured 40 h post infection and luciferase activity in non-targeting (NT) control siRNA transfected HeLa cells was set as 1. Data presented are mean ± SD from three independent experiments (n = 3). d, Knockdown efficiency of siRNAs used in (c). Hela cells were transfected with indicated siRNAs targeting histone methyltransferases and mRNA levels of siRNA targeted genes were measured by RT-qPCR. mRNA levels in non-targeting (NT) control siRNA transfected HeLa cells was set as 1. Data presented are mean ± SD from three independent experiments (n = 3).