Figure 2.

Knockdown of LIPH expression level restrained cell colony formation, proliferation, migration, and invasion in PTC cell lines.

Notes: (A) In KTC-1 and BCPAP, the relative expression levels of LIPH were significantly knocked down by siRNA (si-LIPH) in comparison with the negative control group (si-NC) according to the qRT-PCR (two-tailed Student’s t-test, **P<0.01, ***P<0.001). (B) The corresponding protein expression levels of LIPH knockdown by siRNA in KTC-1 and BCPAP. (C) Colony formation assay: S1 and S2 formed fewer colonies than NC. (D) The columns represent the mean colony numbers for each group from at least three independent experiments, and the vertical bars on top of the columns represent SD (two-tailed Student’s t-test, **P<0.01, ***P<0.001). (E, F) CCK-8 assay: cell proliferation was significantly restrained in S1 and S2 in comparison with NC (two-tailed Student’s t-test, *P<0.05, **P<0.01, ***P<0.001). (G) Transwell migration assay: in both KTC-1 and BCPAP, S1 and S2 had fewer migrating cells than NC. (H) The columns represent the mean migrating cell numbers for each group from at least three independent experiments, and the vertical bars on top of the columns represent SD (two-tailed Student’s t-test, ***P<0.001). (I) Matrigel invasion assay: in both KTC-1 and BCPAP, S1 and S2 had fewer invading cells than NC. (J) The columns represent the mean invading cell number for each group from at least three independent experiments, and the vertical bars on top of the columns represent SD (two-tailed Student’s t-test, ***P<0.001). Magnification ×400.

Abbreviations: CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LIPH, lipase H; PTC, papillary thyroid carcinoma; NC, non-specific siRNA control group; qRT-PCR, quantitative reverse transcription PCR; S1, S2, experimental groups; si-LIPH, LIPH with siRNA knockdown.