Figure 4.

HIF-1α regulated the protein expression levels of LIPH, Slug, and E-cadherin, and the protein expression level of LIPH regulated Slug and E-cadherin.

Notes: (A) The protein expression level of HIF-1α was obviously overexpressed in KTC-1 and BCPAP hypoxia models. The protein expression levels of LIPH, Slug, N-cadherin, and vimentin were significantly upregulated and E-cadherin was downregulated in KTC-1 and BCPAP hypoxia models in comparison with their negative control groups. (B) The protein expression levels of HIF-1α, LIPH, Slug, N-cadherin, vimentin, and E-cadherin showed the opposite trend in the inhibitor groups in comparison with the hypoxia groups. (C) The influence of LIPH expression on the well-known epithelial–mesenchymal transition pathway molecules Slug, N-cadherin, vimentin, and E-cadherin in KTC-1 and BCPAP was analyzed by Western blot assay. The expression levels of Slug, N-cadherin, and vimentin were positively regulated by LIPH expression, while E-cadherin was negatively regulated by LIPH expression.

Abbreviations: CoCl2, cobalt(II) chloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HIF-1α, hypoxia inducible factor-1α; LIPH, lipase H; NC, negative control; NCG, negative control group; OEG, overexpression group; S1, S2, experimental groups.