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Neutrophil-like differentiation is altered with TMG treatment. NB40 or HL60 cells were induced to differentiate with ATRA ± TMG. Cells were harvested at 48 h, and whole-cell lysates were subjected to immunoblotting (a). Transcription levels of genes CTSD (b) and DEFA1 (c) induced by ATRA treatment were analyzed by qPCR. The experiments were repeated three times. GAPDH was used as an internal control. Error bars represent S.E. Ctrl, control.
