Figure 2.

The amino acid and length determinants of p4 antimicrobial activity. A, the sequence logo was determined for the conservative fragment of 44 chemerin ortholog sequences from the UniRef50 database. The logo spans the region between positions 61 and 119 of the human chemerin sequence. The underlying gray line indicates the location of p4. B, SDS-PAGE analysis of the indicated p4-based peptides. Peptides were separated under reducing and nonreducing conditions (left and right panels, respectively, separated by a molecular mass ladder), followed by Coomassie Blue staining. Data are from one experiment and are representative of three independent experiments. C, the sequence of p4 with highlighted amino acid and length determinants of its antimicrobial activity. Critical amino acids are shown in red. The smallest active monomeric form of p4 analog is shown in bold.