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. 2018 Nov 26;294(4):1312–1327. doi: 10.1074/jbc.RA118.003392

Figure 7.

Figure 7.

SRSF3 is hypophosphorylated at steady state. HEK293 cells were transiently transfected with plasmids encoding Myc-SRSF1WT, Myc-SRSF3WT, Myc-SRSF3(85SA), Myc-SRSF3(118SA), or Myc-SRSF3(128SA), where serines located C-terminal to aa 85, 118, or 128 were all mutated to alanines, respectively. Lysates were collected 24 h post-transfection and subjected to SRPK2-mediated phosphorylation or CIP-mediated dephosphorylation or left untreated. Samples were separated by a 15% Tris-Tricine SDS-polyacrylamide gel followed by Western blotting with α-Myc mAb. The mobility of either Myc-SRSF3(85SA) or Myc-SRSF3(118SA) was similar with or without treatment of SRPK2 or CIP. The mobility of untreated Myc-SRSF1WT, Myc-SRSF3(128SA), or Myc-SRSF3WT is lower than that of the SRPK2-treated sample but higher than that of the CIP-treated sample. The asterisk indicates the presence of a slower-migrating band in the untreated sample of Myc-SRSF3(128SA).