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. 2018 Nov 26;294(4):1312–1327. doi: 10.1074/jbc.RA118.003392

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© 2019 Long et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 9. — Localization study of SRSF3 in HEK293 and HEK(K2X) cells. Each cell line was transfected with pCMV-Myc-SRSF3. pCMV-Tag-2c-FLAG empty vector was transfected to HEK and HEK(K2X) as control. pCMV-Tag-2c-FLAG_SRPK2WT/DM was transfected to HEK(K2X) to generate HEK(K2X)-K2WT and HEK(K2X)-K2DM, respectively. Anti-FLAG antibody and Alexa 594 secondary antibody were used to visualize FLAG-SRPK2WT or FLAG-SRPK2DM. Anti-Myc antibody and Alexa 488 secondary antibody were used to visualize Myc-SRSF3. The percentage of cells containing nuclear speckles was evaluated in three independent experiments for each tested plasmid construct. A total of at least 150 cells were counted for each tested construct. HEK(K2X) has a higher percentage of speckle-containing cells than HEK293. HEK(K2X)-K2WT shows significantly fewer speckle-containing cells, when compared with HEK(K2X)-K2DM, which has a percentage similar to that of HEK(K2X). Results were analyzed with Student's t test and presented as mean ± S.D. (error bars) of three independent experiments. *, p < 0.05; **, p < 0.01.