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. 2018 Dec 6;294(4):1240–1249. doi: 10.1074/jbc.RA118.006045

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© 2019 Patel et al.

Published by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

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Figure 1. — Ub^B stimulates cIAP1R-catalyzed Ub transfer. A, nonreduced autoradiograms of lysine discharge reactions showing the disappearance of UbcH5B variant∼³²P-Ub over time in the presence and absence of UbΔGG (300 μm) catalyzed by cIAP1R. B, nonreduced SDS-PAGE showing the cIAP1R-mediated discharge of fluorescently labeled UbcH5B∼Ub to l-lysine over time in the presence of UbΔGG (20 μm) or UbcH5B S22R,F62A,P95D–Ub (20 μm) visualized with a LI-COR Odyssey scanner (top) followed by staining with InstantBlue (bottom). *, fluorescently labeled Ub.