Differential glycan binding of MGLshort H259T
The CFG glycan array data of the MGLshort and MGLshort H259T (200 and 2 μg/ml) were analyzed using the data mining GlycoPattern software (https://glycopattern.emory.edu)7 (18). Listed are glycans that failed to bind MGLshort H259T and showed binding of >10,000 relative fluorescence units for MGLshort. Yellow squares, GalNAc; blue squares, GlcNAc; yellow circles, galactose; red triangles, fucose; purple diamonds, sialic acid; blue circles, glucose; green circles, mannose.