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Loss of PCT1 or ALE1 has minor effects on PC species profile. The indicated strains were grown to late log phase, the cultures were harvested, and the lipids were extracted. PC species were separated and analyzed as described under “Experimental procedures.” The data represent averages of three independent cultures ± S.D. A two-way analysis of variance was performed to determine significance for each strain compared with the WT. *, p ≤ 0.05; **, p ≤ 0.004; ***, p ≤ 0.0001.
