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Gpc1 is expressed in logarithmic phase. WT strain was grown in medium lacking inositol. The cultures were harvested at log (14 h), early stationary (24 h), and late stationary (48 h) phases. Western blotting analysis was performed using anti-HA mouse IRdye 680 and goat anti-mouse IRdye 800. Glucose-6-phosphate dehydrogenase was used as a loading control. The experiment was performed in biological triplicate with identical results (no detectable expression in stationary phase).
