Figure 2.

Mesenchymal stem cells (MSC)‐derived exosomes delivered miR‐125b into vascular smooth muscle cells (VSMCs). (A) Relative expression levels of miR‐125b in MSCs and MSC‐derived exosomes as determined by RT‐qPCR. Three independent experiments (n = 3). (B) Expression levels of miR‐125b in VSMCs with and without treatment with exosomes as determined by RT‐qPCR. *P < 0.05 vs untreated cells. Three independent experiments (n = 3). (C) Exosomes were isolated from MSCs transfected with Cy3‐miR‐125b for 48 h and incubated with VSMCs for 3 h. Localisation of Cy3‐miR‐125b (red) in the exosome‐treated VSMCs was observed using a confocal microscope. Nuclear counterstaining was performed with DAPI (blue)