Figure 2.

Leonurine prevents encephalitogenic T cell infiltration into the CNS in EAE mice. MNCs were isolated from the CNS of naive mice, vehicle‐treated EAE mice and leonurine‐treated EAE mice on day 19 post immunization. (A) Cells were analysed for expression of CD4 and CD8 in the lymphocyte gate by flow cytometry. The percentages and absolute numbers of CD4⁺ and CD8⁺ cells in the CNS were shown (n = 5). (B) The pathogenic IFN‐γ‐ and IL‐17‐producing CD4⁺ T cells were analysed by flow cytometry, and the percentages and absolute numbers in the CNS were shown (n = 5). Statistical significance indicated as *P < 0.05