Figure 4.
Knockdown of CD147 in HNSCC cells reduced tumor growth in vivo. (A) A total of 1 × 106 HN6 negative control cells and shCD147 cells in 100 μL fresh DMEM medium were subcutaneously injected into the left or right flank of the BALB/C nude mice. Tumor volume of two groups are presented. **P < 0.01, based on Student's t test. (B) Tumor weight of two groups are presented. *P < 0.05, based on Student's t test. (C) Tumor growth 20 days after injection of the BALB/C nude mice are shown. (D) Mice were killed and the tumors are isolated. (E) Representative H&E staining of tumor tissues is shown. (F) Immunohistochemical staining of Ki67 and CD147 in shNC and shCD147 xenografted tumors is shown. ***P < 0.001, ****P < 0.0001, based on Student's t test