Figure 3.

UPARANT effects on αvβ3 integrin and Rac‐1. (A) Representative blots showing protein levels of pβ3 integrin (Tyr⁷⁷³), αvβ3 integrin and Rac‐1. β‐actin was used as the loading control. (B‐D) Densitometric analysis showing that UPARANT at 8 mg/kg, but not at 1 mg/kg, reduced up‐regulated levels of pβ3 integrin (Tyr⁷⁷³) and αvβ3 integrin without affecting Rac‐1 levels. (E) Up‐regulated Rac‐1 activity was unaffected by UPARANT at 1 mg/kg, but was decreased by UPARANT at 8 mg/kg (*P < 0.05 and **P < 0.001 vs control; ^§ P < 0.01 and ^§§ P < 0.001 vs STZ; one‐way ANOVA followed by Newman‐Keuls’ multiple comparison posttest). Data are presented as scatter plots (B‐D) or histograms (E). Each plot or histogram represents the mean ± SEM of data from three independent samples