Table 1.
Primer sequences used for quantitative PCR analysis
| Gene | Full name | Forward primer (5′‐3′) | Reverse primer (5′‐3′) |
|---|---|---|---|
| F2r | Protease‐activated receptor 1 | GTTGATCGTTTCCACGGTCT | ACGCAGAGGAGGTAAGCAAA |
| Aqp1 | Aquaporin 1 | AGGCTTCAATTACCCACTGGA | GTGAGCACCGCTGATGTGA |
| Vim | Vimentin | GCTGCGAGAGAAATTGCAGGA | CCACTTTCCGTTCAAGGTCAAG |
| Acta2 | smooth muscle‐α‐actin | TCCCTGGAGAAGAGCTACGAACT | GATGCCCGCTGACTCCAT |
| Tjp1 | Zona Occludens 1 | GCCGCTAAGAGCACAGCAA | GCCCTCCTTTTAACACATCAGA |
| Fn1 | Fibronectin | ATGTGGACCCCTCCTGATAGT | GCCCAGTGATTTCAGCAAAGG |
| Col1a1 | Collagen I | GCTCCTCTTAGGGGCCACT | CCACGTCTCACCATTGGGG |
| Adgre1 | F4/80 | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
| Tnf | Tumor necrosis factor α | CTGTAGCCCACGTCGTAGC | TTGAGATCCATGCCGTTG |
| Ccl2 | Macrophage chemoattractant protein 1 | CATCCACGTGTTGGCTCA | GATCATCTTGCTGGTGAATGAGT |
| IL1b | Interleukin 1β | TGAGCACCTTCTTTTCCTTCA | TTGTCTAATGGGAACGTCACAC |
| Il6 | Interleukin 6 | GCTACCAAACTGGATATAATCAGGA | CCAGGTAGCTATGGTACTCCAGAA |
| Tgfb1 | Transforming growth factor β1 | CTGACCCCCACTGATACGCCT | TGCTGTCACAAGAGCAGTGAGC |
| Tgfb3 | Transforming growth factor β3 | CTGTTGAGGAGAGAGTCCAACTTG | CCAGTATGTCTCCATTGGGCTGA |
| Cxcl1 | KC | ATAATGGGCTTTTACATTCTTTAACC | AGTCCTTTGAACGTCTCTGTCC |
| Tbp | Tata‐binding protein | CCTTGTACCCTTCACCAATGAC | ACAGCCAAGATTCACGGTAGA |
| Gapdh | Glyceraldehyde 3‐phosphate dehydrogenase | CTCATGACCACAGTCCATGC | CACATTGGGGGTAGGAACAC |
| Actb | β‐actin | GTGACGTTGACATCCGTAAAGA | GCCGGACTCATCGTACTCC |