Figure 4.

Reduced osteoclastic differentiation is associated with reduced levels of MMPs and increased level of KLF2. Total RNA was isolated from murine monocytic cell (RAW 264.7) (A, C) and murine primary bone marrow cells (B, D) that were subjected to osteoclastic differentiation in the presence or absence of HDACi, such as SAHA (10 nmol/L), MS275 (10 nmol/L) or TSA (10 nmol/L), and were subjected to real‐time RT‐PCR analysis for expression levels of MMP3, 9 and 13 along with KLF2 keeping GAPDH as an internal control, shown graphically. RT‐PCR experiments were performed in triplicate. The experiment was performed at least three times, and (*) indicates statistical significance (P < 0.05) when compared to the respective controls. (E) Western blot analysis was performed during the course of osteoclastic differentiation of RAW 264.7 cells for KLF2, NFκB (p65) and MMP9 proteins keeping GAPDH as an internal control. (F) Western blot analysis was performed during the course of osteoclastic differentiation of RAW 264.7 cells as indicated for KLF2, NFκB (p65) and MMP9 proteins keeping GAPDH as an internal control in the presence or absence of HDACi