Figure 5.

HDAC inhibitors reduce K/BxN serum‐induced arthritic inflammation, and bone and cartilage damage in mice. Group of C57BL/6 mice (8‐10 mice/group) were induced for development of arthritic inflammation using K/BxN serum (control), and were subjected to i.p. injection of HDACi (experimental; either SAHA, 10 mg/kg body weight; or SAHA 30 mg/kg body weight; or MS275, 5 mg/kg body weight) during induction of arthritis for 7 days. (A) Measured ankle inflammations are shown graphically during the development of arthritis for each inhibitor separately, and (*) indicate statistical significance (P < 0.05) when compared to the respective controls. (B) Haematoxylin and eosin staining of ankles (representative of four animals of each group) showed that reduced bone and cartilage damage after treatment with either of the HDAC inhibitors (SAHA or MS275). Arrowheads indicate the damaged area of the joints. (C) Clinical score of the histology showed that a damage of bone and cartilage were significantly reduced after treatment with SAHA or MS275 inhibitors