Figure 1.

O‐GlcNAcylation and DDX5 are significantly up‐regulated in human colorectal cancer tissues and cell lines. A, Representative images of immunohistochemistry (IHC) staining of O‐GlcNAcylation and haematoxylin and eosin (HE) staining in 90 CRC tissues and 90 adjacent normal tissues. The high (upper) and low (lower) expression levels of O‐GlcNAcylation were evaluated by semi‐quantitative staining intensity (high score: 8‐12; low score 0‐6). B, Representative images of immunohistochemistry (IHC) staining of DDX5 and haematoxylin and eosin (HE) staining in 90 CRC tissues and 90 adjacent normal tissues. The high (upper) and low (lower) expression levels of O‐GlcNAcylation were evaluated by semi‐quantitative staining intensity (high score: 8‐12; low score 0‐6). C and D, Chi‐square analysis of O‐GlcNAcylation and DDX5 levels in 90 CRC tissues and 90 adjacent normal tissues. E and F, the Kaplan‐Meier curve depicts the overall survival of patients with CRC (n = 90). The curves were stratified based on O‐GlcNAcylation and DDX5 levels, respectively. The overall survival was defined as the interval between the date of surgery and death or the last follow‐up. G, The correlation coefficient (rs) between the scores of DDX5 and O‐GlcNAcylation was determined in the CRC tissue (r ² = 0.8927). H, Kaplan‐Meier analysed concurrent O‐GlcNAcylation and DDX5 expression with recurrence and overall survival in CRC (n = 90) patients. I and J, O‐GlcNAcylation and DDX5 expression in established colonic epithelial cells (NCM460) and colon cancer cell lines were separately detected by WB. The levels of DDX5 and O‐GlcNAcylation were normalized to the level of GAPDH. The data are shown as the means + SD from three independent experiments (including WB). *P < 0.0001 indicates statistical significance. K, Knockdown of OGT can inhibit the level of DDX5, and overexpression of OGT increases the level of DDX5. The data are shown as the means + SD from three independent experiments (including WB)