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. 2018 Nov 5;23(2):865–876. doi: 10.1111/jcmm.13986

Figure 2.

Figure 2

Knockdown of GAS6‐AS2 suppressed bladder cancer proliferation via induction of G1 phase arrest. A, GAS6‐AS2 were knockdown in both T24 and 5637 cells by two shRNA vectors. B, GAS6‐AS2 knockdown inhibited proliferation of T24 and 5637 cell lines. C, GAS6‐AS2 knockdown inhibited colony formation abilities of T24 and 5637 cell lines. D, GAS6‐AS2 knockdown contributed G1 phase cell cycle arrest in both cell lines. E, EDU assay confirmed reduced proliferation abilities after GAS6‐AS2 knockdown. F, GAS6‐AS2 knockdown altered cell cycle proteins. Data represent means ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001