Figure 5.

GAS6‐AS2 specifically sponged miR‐298. A, Detection of subcellular location of GAS6‐AS2 in both T24 and 5637 cell lines. B, The predicted targeting sequence of miR‐298 on the GAS6‐AS2. C, The correlation between GAS6‐AS2 and miR‐298 expression based on TCGA database. D, Construction of GAS6‐AS2 wild‐type and mutant type luciferase vectors. E, The wild‐type and mutant type of binding sites and luciferase reporter assay in HEK293T cells demonstrated combination between miR‐298 and GAS6‐AS2. F, Anti‐Ago2 RIP assay verified the combination between miR‐298 and GAS6‐AS2. G, Effect of GAS6‐AS2 knockdown or overexpression on miR‐298 expression. Data represent means ± SD of at least three independent experiments. **P < 0.01, ***P < 0.001