Figure 2.

The expression of HOXA‐AS2 is downregulated upon osteogenic induction. A, The effect of HOXA‐AS2 on calcium deposition in MenSCs, determined by Alizarin Red S staining. B, The alkaline phosphatase activity was examined in MenSCs by BCIP/NBT liquid substrate system, with or without HOXA‐AS2 knockdown. C, The effect of HOXA‐AS2 on osteogenic marker gene expressions in MenSCs, measured by qRT‐PCR. All values are the average of at least three biological replicates and data shown are the mean ± SD. *P < 0.05, **P < 0.01 vs shGFP