Figure 3.

HOXA‐AS2 regulates the transcriptional activity of NF‐κB. A, The effect of Bay 11‐7082 on calcium deposition in MenSCs, determined by Alizarin Red S staining. B, The effect of Bay 11‐7082 on alkaline phosphatase activity in MenSCs, examined by BCIP/NBT liquid substrate system. C, The expressions of osteogenic marker genes were examined by qRT‐PCR in control and HOXA‐AS2‐depleted MenSCs respectively. D, Western blot analysis showing the effect of HOXA‐AS2 on acetylation status of RelA K310 site in MenSCs with or without HOXA‐AS2 knockdown, and then quantified using ImageJ software. All values are the average of at least three biological replicates and data shown are the mean ± SD. *P < 0.05, **P < 0.01 vs shGFP