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Transfection of MSCs with AAV vector. (A) Representative photomicrograph of MSCs transfected with AAV‐NC‐siRNA/GFP, scale bar: 100 μm. (B) FACS analysis showing the transfection efficiency of GFP transfected MSCs. (C) qRT‐PCR analysis of MR mRNA expression in MSCs 48 h after transfection with AAV‐MR‐siRNA/GFP. (D) AAV‐siRNA/GFP transfection did not affect cell viability examined with CCK‐8 assay. All data were collected from three independent experiments. *P < 0.05 vs control and NC
