Figure 2.

Effects of phosSNP rs227584 on protein phosphorylation (A) substrate‐kinase interaction assay for C17orf53 and NEK2 proteins. Two representative Western blots were presented. Samples were Co‐IP products of cell lysates from MG63 cells which was transiently transfected with variant, mutant, or wild‐type C17orf53 genes, respectively. The Co‐IP product was prepared with anti‐NEK2 antibody. (B) Allele‐specific effects of rs227584 on C17orf53 total protein phosphorylation level. Presented are estimated phosphate levels in the immunoaffinity‐purified C17orf53 proteins (mean and SD). The phosphate level under the condition of variant type C17orf53 transfection (A120P126) was scaled as 1.0. *P < 0.05, as compared with the variant type. NS: insignificant