Table 2.
Target Gene Data.
| Gene | Forward primer 5’---3’ | Reverse primer 5’---3’ | Annealing/extension temp (°C) | GenBank accession number |
|---|---|---|---|---|
| VEGF | CTTGCTGCTCTACCTCCACCAT | ATGATTCTGCCCTCCTCCTTCT | 62 | AB021221 |
| PGF | CCTACGTGGAGCTGACGTTCT | TCCTTTCCGGCTTCATCTTCT | 60 | S72960 |
| sFlt1 | GGGAAGAAATCCTCCAGAAGAAAGA | GAGATCCGAGAGAAAACAGCCTTT | 64 | AF063657 |
| PRB | TCGGACACCTTGCCTGAAGT | CAGGGCCGAGGGAAGAGTAG | 60 | NM_000926.4 |
| ESR1 | AGATCTTCGACATGCTGCTGGCTA | AGACTTCAGGGTGCTGGACAGAAA | 67.6 | X03635 |
| hCGβ | CTACTGCCCCACCATGACCC | TGGACTCGAAGCGCACATC | 63.2 | J00117.1 |
| hPL | GCATGACTCCCAGACCTCCT | TGCGGAGCAGCTCTAGATTGG | 63.2 | J00118.1 |
| CYP24A1 | CATCATGGCCATCAAAACAAT | GCAGCTCGACTGGAGTGAC | 60 | NM_000782.4 |
| CYP27B1 | CACCCGACACGGAGACCTT | TCAACAGCGTGGACACAAACA | 60 | NM_000785 |
| GRα (NR3C1) | CTTGGATTCTATGCATGAAGTGGTT | TTGGAAGCAATAGTTAAGGAGATTTTC | 61.6 | NM_000176.2 |
| VDR | TGGCTTTCACTTCAATGCTATGA | CGTCGGTTGTCCTTGGTGAT | 62 | NM_000376.2 |