Figure 2.

Knockdown of PRMT5 suppresses proliferation of lung cancer cells. (A, B) A549 and H1299 cells were infected with lentivirus containing PRMT5 and scramble (scr) shRNA and PRMT5 mRNA expression level was measured by qRT‐PCR. *P < 0.05 vs scr. (C, D) A549 and H1299 cells were infected with lentivirus containing PRMT5 and scramble (scr) shRNA and the PRMT5 protein expression level was detected by Western blotting. (E, F) Quantitative analysis of PRMT5 protein expression level in A549 and H1299 cells. *P < 0.05 vs scr. (G, H) A549 and H1299 cells were infected with lentivirus containing PRMT5 and scramble (scr) shRNA and the cell proliferation were measured by CCK‐8 assay at the indicated time points. *P < 0.05 vs scr. (I) The cyclin E1 and cyclin D1 expression level was detected by Western blotting when PRMT5 was down‐regulated in A549 and H1299 cells. (J, K) Quantitative analysis of cyclin E1 and cyclin D1 protein expression level in A549 and H1299 cells. *P < 0.05 vs scr