Figure 2.

Conditioned medium from hAMSC (CM‐hAMSC) treatment restores motor symptoms in R6/2 mice. A, The development of the hind‐limb clasping phenotype in wild‐type (WT) mice treated with saline, and R6/2 mice treated with saline, control medium (CTRL) or CM‐hAMSC. One way ANOVA with repeated measurements indicated a statistically significant effect of treatment with CM‐hAMSC (P < 0.00002) at 12 and 13 weeks of age versus R6/2 treated with saline. B, Latency to fall from the accelerating rotarod in the R6/2 mice. A one way ANOVA indicated that R6/2 mice treated with saline exhibited a progressive decrease in the latency to fall and this decrease was blunted by CM‐hAMSC treatment. C, Total distance travelled during a 10 min test session in an open field test. The CM‐hAMSC treated R6/2 mice had a lesser decrease in distance travelled as compared to the saline treated R6/2 mice in the late stage (12‐13 weeks of age) of disease (P < 0.00002). *vs R6/2 + saline; § vs R6/2 + CTRL