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. 2018 Dec 25;23(2):1581–1592. doi: 10.1111/jcmm.14113

Figure 4.

Figure 4

Double‐label immunofluorescence for Neurotrace and neuronal intranuclear inclusions (NIIs). A, The double label immunostaining was performed in wild‐type mice, and in saline‐, CTRL‐ and conditioned medium from hAMSC (CM‐hAMSC)‐treated R6/2 mice. Neurotrace was used to label all neurons in the striatum and EM48 to stain the intranuclear NIIs (visualized in red fluorescence). Neuronal intranuclear inclusions were absent in wild‐type mice. (B,C) Graphs show the effect of CM‐hAMSC on NIIs number and size. The one‐way repeated measures ANOVA performed on data obtained by each group revealed a statistically significant effect of treatment on NIIs size (C, *** P < 0.0001)