Figure 2.

Subcellular localization of claudin 1 in human breast cancer cell lines. (A) IF microscopy was used to detect claudin 1 in breast cancer cell lines. The adheren junction protein E-cadherin was used as a positive control for membrane staining, and the MDCKII cells were used as a positive control for claudin 1 membrane localization. (B) Western blot analysis showing relative levels of claudin 1 in different subcellular fractions from two representative cell lines. SRA (steroid receptor RNA activator) and E-cadherin were used to confirm cytoplasmic (C) and membrane (M) fractions, respectively. Scale bars = 20μm.