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Activation of PKC by TPA in MCF7 cells led to the upregulation of claudin 1 expression and increased protein level in a time-dependent manner. Real-time PCR and Western blot analyses were carried out on MCF7 cells treated with TPA (100 nM TPA for up to 18 hours, as described in Materials and Methods). PKC activation led to (A) a large fold increase in claudin 1 transcript and (B) protein levels (n = 3 experimental replicates).
