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PKC increases accumulation of claudin 1 protein in the cytoplasm of MCF7 cells. (A) IF with the claudin 1 antibody was used to show TPA treatment increased accumulation of endogenous claudin 1 in the cytoplasm of MCF7 cells. (B, C) Exogenous GFP-tagged claudin 1, visualized using a Zeiss Axio Observer D1 inverted microscope, was also increased in the cytoplasm following TPA treatment. Scale bars = 20 μm.
