Fig 1. BD FACSLyric clinical evaluation flowchart.

T-, B-, and NK cells were stained with BD Multitest reagents for testing of the 4 protocols depicted from the left to the right side: 1. Method comparison; 2. Inter-laboratory variability; 3. Determination of the reference intervals and 4. Evaluation of the specimen and stained sample storage. The summary for each protocol includes the number of specimens enrolled and cohort of origin, followed by the instrument(s) used for acquisition of the stained sample.