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. 2018 Dec 3;23(2):1553–1561. doi: 10.1111/jcmm.14063

Figure 2.

Figure 2

RNF168 depletion inhibits cell proliferation and invasion in oesophageal cancer cells. (A) RNF168 depletion effect by two different siRNA oligos. NEC cell were transfected with siRNF168 or siControl. After 48 h, RNF168 mRNA levels are determined by real‐time PCR with 36B4 as internal control. (B, C) The WST‐1 assay was used to determine the cellular metabolic activity at indicated time points after transfection. NEC and EC109 cells were transfected with siRNF168 and siControl. After 24 h, cells were seeded into 96‐well plates. These experiments were done in triplicates. All values are mean ± SD (n = 3, *P < 0.05; **P < 0.01, ***P < 0.001). (D, E) Transwell invasion assay of NEC cells transfected with indicated RNF168 siRNA. The cell number is counted and data are presented as ±SD. **, P < 0.01, ***, P < 0.001 (Student's t test). (F, G) Wound healing assay of NEC transfected with the indicated siRNA. Quantification of wound closure at the indicated time points. Data are presented as ±SD. **, P < 0.01, ***, P < 0.001 (Student's t test)